0 Replies
Cellular internalization of quantum dots.
Methods Mol Biol. 2013;991:249-59
Authors: Huang YW, Lee HJ, Liu BR, Chiang HJ, Wu CH
Abstract
Cell-penetrating peptides (CPPs) can facilitate uptake of quantum dots (QDs) for a variety of basic and applied sciences. Here we describe a method that utilizes simple noncovalent interactions to... Contine Reading & Discuss→
0 Replies
In Vivo Target Validation by Inducible RNAi in Human Xenograft Mouse Models.
Methods Mol Biol. 2013;986:325-37
Authors: Mazzoletti M, Texidó G
Abstract
Proper target selection and validation are crucial to the discovery of new anti-cancer agents. Since tumors depend on a suitable microenvironment for their growth, once a putative target... Contine Reading & Discuss→
0 Replies
Target Validation in Mice by Constitutive and Conditional RNAi.
Methods Mol Biol. 2013;986:307-23
Authors: Kleinhammer A, Wurst W, Kühn R
Abstract
Gene silencing by RNA interference (RNAi) has become a standard method for the characterization of gene function in mammalian cells. Short hairpin (sh) RNAs expressed from stably integrated vectors... Contine Reading & Discuss→
0 Replies
Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development.
Methods Mol Biol. 2013;986:97-104
Authors: Drosopoulos K, Linardopoulos S
Abstract
Cellular models for siRNA and small molecule high throughput screening have been widely used in the last decade to identify targets for drug discovery. As an example,... Contine Reading & Discuss→
0 Replies
High-Throughput RNAi Screening for the Identification of Novel Targets.
Methods Mol Biol. 2013;986:89-95
Authors: Henderson MC, Azorsa DO
Abstract
Gene silencing through RNA interference has provided researchers with an effective way to study gene function. High-throughput RNA interference (HT-RNAi) screening has further permitted researchers to identify functionally relevant mediators of... Contine Reading & Discuss→
0 Replies
Western Blot Evaluation of siRNA Delivery by pH-Responsive Peptides.
Methods Mol Biol. 2013;986:73-87
Authors: Liang W, Mason AJ, Lam JK
Abstract
Gene silencing, via RNA interference (RNAi) technologies using small interfering RNA (siRNA), has been developed as an important tool for target identification and validation in drug discovery and has... Contine Reading & Discuss→
0 Replies
Related Articles
The Use of VIGS Technology to Study Plant-Herbivore Interactions.
Methods Mol Biol. 2013;975:109-37
Authors: Galis I, Schuman MC, Gase K, Hettenhausen C, Hartl M, Dinh ST, Wu J, Bonaventure G, Baldwin IT
Abstract
Plants employ a large variety of defense strategies to resist herbivores, which require transcriptional reprogramming... Contine Reading & Discuss→
0 Replies
Related Articles
Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing.
Methods Mol Biol. 2013;975:15-32
Authors: Ramanna H, Ding XS, Nelson RS
Abstract
The exploding availability of genome and EST-based sequences from grasses requires a technology that allows rapid functional analysis of the... Contine Reading & Discuss→
0 Replies
Related Articles
Pooled shRNA Screenings: Experimental Approach.
Methods Mol Biol. 2013;980:353-70
Authors: Rodriguez-Barrueco R, Marshall N, Silva JM
Abstract
RNA interference (RNAi) has emerged as a powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. In the last years, several arrayed shRNA libraries that can silence almost... Contine Reading & Discuss→
0 Replies
Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors.
Methods Mol Biol. 2013;960:401-9
Authors: Satoh T, Manel N
Abstract
Monocyte-derived dendritic cells (MDDCs) are widely used in the field of human immunology. Although a variety of gene delivery procedures have been used in MDDC, it has remained difficult to... Contine Reading & Discuss→
